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KMID : 0379119870150020125
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Korean Journal of Mycology
1987 Volume.15 No. 2 p.125 ~ p.125
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Study of the Immobilization of Candida rugosa Lipase for Fat Splitting in Reverse - phase System
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Kang Sung-Tae
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Abstract
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For the effective enzymatic hydrolysis of olive oil, the potential of reverse-phase as new reaction system was tried. Lipase was immobilized by adsorption in macroporous organic synthetic resin, Amberlite XAD-7, and the catalytic reaction of immobilized lipase was performed on a reverse-phase system which was composed of water-insoluble substrates and immobilized enzyme dispersed in the nonpolar solvents. The optimum conditions for lipase immobilization such as immobilization times and amount of enzyme to be attached to the resin were investigated. Isooctane was found to be the most effective organic solvent of the nine types of organic solvents tested for the hydrolysis of olive oil. The immobilized enzyme did not obey Michaelis-Menten kinetics and optimal temperature and pH of reaction were 35℃ and pH 6.0, respectively. The enzyme reaction rate was affected by stirring rates. Batch hydrolysis of olive oil by lipase in reverse-phase has been also carried out. Olive oil(10%, v/v) can be completely hydrolyzed within 5hr.
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